THE FOLLOWING SHOWS THE GENOTYPE OF A PARTIAL DIPLOID BACTERIAL CELL – WHERE ONE CHROMOSOMAL REGION CONTAINING THE LAC OPERON IN E,COLI IS GIVEN, AND THE OTHER FRAGMENT IS FROM A PLASMID CARRYING ANOTHER LAC OPERON FROM ANOTHER SOURCE. THE TWO ARE SEPARATED BY A SLASH (/).
THE POSSIBLE ANSWERS INDICATE WITH A ʺ+ʺ OR A ʺ-ʺ WHETHER Β-GALACTOSIDASE WOULD BE EXPECTED TO BE PRODUCED AT INDUCED LEVELS UNDER TWO CIRCUMSTANCES: 1) FIRST IN THE ABSENCE OF LACTOSE AND 2) SECOND IN THE PRESENCE OF LACTOSE. (ASSUME THAT GLUCOSE IS NOT PRESENT IN THE MEDIUM.) GENOTYPE H: IS OC Z-/ Fʹ IS O+ Z+ KEY: I+ = WILD-TYPE REPRESSOR I- = MUTANT REPRESSOR (UNABLE TO BIND TO THE OPERATOR) IS = MUTANT REPRESSOR (INSENSITIVE TO LACTOSE) O+ = WILD-TYPE OPERATOR OC = CONSTITUTIVE OPERATOR (INSENSITIVE TO REPRESSOR)
The following shows the genotype of a partial diploid bacterial cell – where one chromosomal region containing the lac operon in E,coli is given, and the other fragment is from a plasmid carrying another lac operon from another source. The two are separated by a slash (/). The possible answers indicate with a ʺ+ʺ or a ʺ-ʺ whether β-galactosidase would be expected to be produced at induced levels under two circumstances: 1) first in the absence of lactose and 2) second in the presence of lactose. (Assume that glucose is not present in the medium.) Genotype H: Is Oc Z-/ Fʹ Is O+ Z+ KEY: I+ = wild-type repressor I- = mutant repressor (unable to bind to the operator) Is = mutant repressor (insensitive to lactose) O+ = wild-type operator Oc = constitutive operator (insensitive to repressor)
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