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31 Jul

Estimate the concentration of substrate at the surface


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The values of and for an enzyme (21°C and pH 7.1) are 0.004 kmol/m3 and 10 kmol/m3 s, respectively. We immobilized this enzyme by attaching it covalently to acrylamide-based polymers that can be assumed to have spherical shape (diameter = 1 mm). The effectiveness of the immobilized enzyme was found to be 70 percent of the free enzyme when the concentration of the substrate was 0.5 kmol/m3 .The reaction was carried out in a stirred reactor with an agitation speed of 50 rpm. (a) Estimate the concentration of the substrate at the surface of the immobilized enzyme, (b) Estimate ks a.

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